Eppendorf 5402 Manual Updated |best| Jun 2026

Most Eppendorf 5402 fixed-angle aluminum rotors can be autoclaved at 121°C for 20 minutes to eliminate biological contamination. Always remove the rotor lid seals before autoclaving unless specified by updated vendor documentation. Defrosting the Refrigeration System

Shows the set speed or current running speed.

120V / 230V options (dependent on regional model variants), 50–60 Hz 2. Interface and Control Panel Overview

To maximize sample yield and protect the centrifuge motor, follow these precise steps for every run. Step 1: Pre-Cooling (For Refrigerated Runs)

Load your tubes symmetrically. A balanced load is mandatory to prevent dangerous vibrations and automated emergency shut-offs. eppendorf 5402 manual updated

Leave the centrifuge lid open slightly when not in use to allow condensation to evaporate. This prevents corrosion and mold growth inside the cooling chamber. Weekly Rotor Care Remove the rotor completely from the centrifuge shaft.

Understanding the physical limits of your equipment prevents motor damage and sample loss. The Eppendorf 5402 delivers precise sedimentation of standard microcentrifuge tubes through the following parameters: 14,000 revolutions per minute (rpm). Maximum RCF: 16,000 × g (Relative Centrifugal Force).

Always load the rotor symmetrically to prevent imbalance, which can cause severe mechanical damage.

Standard F-45-18-11 rotor accommodates 18 tubes (1.5 mL or 2.0 mL) at a 45∘45 raised to the composed with power Most Eppendorf 5402 fixed-angle aluminum rotors can be

Available in 120V (60 Hz) and 230V (50-60 Hz) configurations 2. Interface and Control Panel Overview

Commences or manually terminates the centrifugation cycle.

Understanding the fundamental capabilities of the Eppendorf 5402 ensures you operate the unit within its intended limits, protecting both the operator and the samples. Key Specifications 14,000 RPM (Revolutions Per Minute).

Search the official Eppendorf Technical Support forum using the tag #Eppendorf5402Update or contact their legacy equipment team directly – they still support this legendary centrifuge. 120V / 230V options (dependent on regional model

This usually indicates a fault with the temperature sensor or a refrigeration fluid leak. Turn off the machine, unplug it from the wall, and contact a certified technician. 6. Finding and Downloading the Updated Manual

This denotes (Deutsche Emissions). It was added to the updated manual in 2019 to reflect stricter EU noise directives (max 62 dBA for the 5402). No operational change is required.

The 5402 is optimized for temperature-sensitive samples, with a cooling range of -4°C to 40°C GMI - Trusted Laboratory Solutions Maximum Speed: 14,000 RPM (approximately 16,000 x g). Speed Adjustment: Adjustable in 100 RPM increments. Timer Range: 0 to 99 minutes, with a dedicated Short Spin button for rapid pulses. Typically accommodates up to 18 x 1.5/2.0 mL tubes Control Panel: