Narishige Pc10 Manual New [360p - FHD]
The glass capillary must sit perfectly centered inside the platinum loop. If the capillary leans closer to one side of the coil, one side of the glass will melt faster, resulting in bent or hooked pipette tips. Loosen the coil mounting screws to manually center the loop if necessary. Troubleshooting Matrix Probable Cause Corrective Action Off-center heating coil or uneven airflow. Center the coil; shield the unit from drafts. Glass breaks instantly Heater setting is too low; weight is too heavy. Increase the heater dial value incrementally. No heat generated Burned-out platinum coil or blown fuse. Inspect the coil for breaks; check the rear fuse. Inconsistent tip sizes Variations in glass OD or sliding friction. Clean the vertical guide rods with a dry cloth.
The device uses gravitational force as its pulling mechanism, which can be adjusted through several manual and digital controls:
Adjust the dial to a slightly lower or higher setting depending on the desired taper smoothness.
Toggles between single-stage pulling and two-stage pulling. narishige pc10 manual new
Select the two-stage mode for precise control over the taper length and tip diameter.
The Narishige PC-10 offers a range of benefits to researchers and scientists, including:
Detachable magnetic or screw-on weights applied to the lower slider to control the pulling force. Control Panel Interface Power Switch: Turns the unit on and off. The glass capillary must sit perfectly centered inside
A key feature that set the PC-10 apart was its two pulling modes: a single-pull and a double-pull. The single-pull mode pulls the glass in one continuous stretch, ideal for longer, thinner needles. The double-pull mode, however, is where the PC-10’s versatility shines. It allows the heating and pulling parameters to be changed mid-process, enabling researchers to produce both long, thin needles for microinjection and robust microelectrodes for patch clamping on the same device. To adjust the pulling tension, the unit comes with four weights (two light and two heavy) that can be attached or removed to fine-tune the gravitational force.
, the PC-10 remains a robust tool for creating tips ranging from sub-micron sizes to tens of microns. Core Functionality and Modes
If you need help setting up parameters for a specific type of experiment, tell me: Increase the heater dial value incrementally
When setting up a new Narishige PC-10, proper installation ensures mechanical calibration remains intact and prevents premature wear on the heating filament. Environmental Requirements
Before first-time use, wipe the vertical guide shaft with a lint-free cloth moistened with a small amount of light machine oil or alcohol to ensure friction-free travel.
Initiates the heating and pulling cycle or resets the sliders. 4. Step-by-Step Operating Instructions
: Two Light Weights (~25g each) and two Heavy Weights (~100g each).
The Narishige PC-10 is a dual-stage vertical glass micropipette puller widely used in electrophysiology, microinjection, and cellular manipulation. This comprehensive guide serves as an operational and technical manual for the PC-10 model, detailing installation, parameter adjustment, and routine troubleshooting. 1. Product Overview and Technical Specifications